Table 1.
Quantities of mouse tissues needed to DNA adduct analysis
Organ | Wet weight yielding 100 μg DNA (mg) | Notes |
---|---|---|
Spleen | 100 | Pool spleens from control mice when spleen weighs <100 mg |
Liver | 50 | Prone to RNA contamination; minimized by doubling amount of RNAse during DNA isolation |
Kidney | 100 | Longer homogenization may be required to obtain high yields |
Colon | 50 | |
Lung | 100 |