Table 1.
Quantities of mouse tissues needed to DNA adduct analysis
| Organ | Wet weight yielding 100 μg DNA (mg) | Notes |
|---|---|---|
| Spleen | 100 | Pool spleens from control mice when spleen weighs <100 mg |
| Liver | 50 | Prone to RNA contamination; minimized by doubling amount of RNAse during DNA isolation |
| Kidney | 100 | Longer homogenization may be required to obtain high yields |
| Colon | 50 | |
| Lung | 100 |