Table 2. Volumes of stock solutions added to DNA hydrolysis cocktail.

Stock Solution of Reagent	Volume Added (μl)
30 mM Sodium acetate (pH 6.8)	30
10 mM ZnCl2 (cofactor for nuclease P1)	10
2 unit/μl Nuclease P1 in sodium acetate (pH 6.8)	2
4000 units/ml DNase I in deionized water	2
20 μM [15N4]-dX	0.5
20 μM [15N3]-dU	0.5
1 μM [15N4]-dI	1
1 μM [15N5]-8-oxo-dG	1
100 nM [15N5]-1,N6-εdA	1
100 nM [15N3]-εdC	1
100 nM [15N5]-1,N2-εdG	1
10 mg/ml Tetrahydrouridine in deionized water	0.5
1 mg/ml Coformycin in deionized water	1
50 mM Desferrioxamine in deionized water	3
100 mM Butylated hydroxytoluene in deionized water	0.5
Total volume	55