. Author manuscript; available in PMC: 2010 Mar 5.

Published in final edited form as: Nat Protoc. 2008;3(8):1287–1298. doi: 10.1038/nprot.2008.119

Table 3. Volumes of stock solutions added to dephosphorylation cocktail.

Stock Solution of Reagent	Volume Added (μl)
30 mM Sodium acetate (pH 7.8)	40
1 mg/ml Coformycin in deionized water	1
17 units/μl Alkaline phosphatase in deionized water	2
100 units/ml Phosphodiesterase I in deionized water	2
Total volume	45