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Acetonitrile gradient for HPLC pre-purification of nucleosides, step 31
| Time (min) | Gradient1 |
|---|---|
| 0 – 45 | 1% to 3.5% |
| 45-50 | 3.5% to 6% |
| 50-55 | 6% to 8% |
| 55-75 | 8% |
| 75-82 | 100% |
| 82-93 | 1% |
Percent acetonitrile in 8 mM ammonium acetate (v/v); flow rate 0.5 ml/min; column temperature 32 °C
