. Author manuscript; available in PMC: 2010 Mar 5.

Published in final edited form as: Nat Protoc. 2008;3(8):1287–1298. doi: 10.1038/nprot.2008.119

Table 5.

Limits of detection, DNA requirements and parameters for preparation of calibration curve samples

Lesion	LOD (fmol)	Minimum DNA (μg)	Unlabeled standard (nM)	Internal standard (nM)
dX	10	50	62.5-1000¹	250
dU	50	50	62.5-1000	250
dI	5	10-20	6.25-100	25
8-oxo-dG	5	50	6.25-100	25
1,N²-εdG	10	100	0.625-10	2.5
εdC	0.5	50	0.625-10	2.5
εdA	1	10-20	0.625-10	2.5

Prepare 5 concentrations differing by 2-fold (e.g., 62.5, 125, 250, 500, 1000)