Table 7.
LC/MS-MS parameters for Agilent 6410 triple quadrupole mass spectrometer
| Mass Spec. Parameters | εdA | εdC | 1,N2-εdG | 8-oxo-dG |
|---|---|---|---|---|
| 14N Q1→Q3 m/z1 | 276→160 | 252→136 | 292→176 | 284→168 |
| 15N Q1→Q3 m/z | 281→165 | 255→139 | 297→181 | 289→173 |
| Gas temperature (°C) | 350 | 350 | 350 | 350 |
| Gas flow (L/min) | 10 | 10 | 10 | 10 |
| Nebulizer gas (psi) | 20 | 20 | 20 | 30 |
| Capillary voltage (V) | 4000 | 4000 | 3500 | 4000 |
| Fragmentor potential (V) | 70 | 70 | 85 | 80 |
| Collision energy (V) | 12 | 12 | 14 | 10 |
| Delta EMV (V) | 700 | 700 | 1000 | 700 |
| Column temperature (°C) | 10 | 10 | 40 | 40 |
| HPLC Parameters2 | εdA | εdC | 1,1,N2-εdG | 8-oxo-dG |
| % Solvent B | 0.8 | 0.8 | 2 | 3 |
| Retention time (min)3 | 19.5-21.5 | 15-17 | 14.5-16.5 | 5.5-7.5 |
1
m/z values monitored in MRM mode for the transition of the parent ion to the fragment ion; 14N refers to the analyte under study; 15N refers to the isotopically labeled internal standard
2
Thermo Hypersil Gold C18 column used in step 39
3
Retention time is expected to range over the values indicated