Figure 2.

RPLC separation of degradation products of Mas-DH at initial concentration of 1 μM incubated with 1-cm pieces of Mt for 1, 5, 60, 90, or 120 min. Chromatographic conditions and identification of peaks are described in the text. Peak identities: 1, Mas-DH + 2O; 2 and 4, Mas-DH + O; 3, Mas-DH-(1–30); 5, Mas-DH-(1–29); and 6, Mas-DH. We observed a run-to-run variation in retention times, likely because of the 20 μl/min flow rate used with the 0.5-mm column. The Michrom UMA is designed for accurate delivery at flow rates of 50 μl/min and higher.