Figure 5.

Strategy to profile the aggregation of platelets with neutrophils or monocytes. Whole blood flow cytometry was performed after samples were stained with specific cell surface markers conjugated with proper fluorochrome as described in “FACS analysis and immunohistochemistry.” The strategy to gate the specific platelet-leukocyte aggregation was described. The first step is to differentiate neutrophils from monocytes with CD14 surface marker, which then further identified with makers for platelets, respectively.