Table 1.

Effect of pharmacologic inhibitors on GPIb-α shedding during platelet storage

Mouse platelets	GPIb-α mean fluorescence (± SEM)	P from stored PRP
Fresh PRPr	421.9 (± 12.6)	< 0.001
Stored PRP (Vehicle)	34.1 (± 5.5)
Stored PRP/PKC inhibitor (Ro 31-8220 2 μg/mL)	28.5 (± 10.9)	n.s.
Stored PRP/ERK inhibitor (PD98059 20μM)	28.2 (± 8.9)	n.s.
Stored PRP/Caspases inhibitor (Z-VAD 20μM)	38.7 (± 9.1)	n.s.

Surface expression of GPIbα was measured by flow cytometry on fresh platelets or platelets stored in presence of inhibitors of PKC (Ro 31-8220), ERK mitogen-activated protein kinase (PD98059), caspases (Z-VAD), or dimethyl sulfoxide (Vehicle). Results are given as mean ± SEM, n = 8.