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. Author manuscript; available in PMC: 2010 Mar 5.
Published in final edited form as: Dev Dyn. 2009 Jun;238(6):1422–1432. doi: 10.1002/dvdy.21931

Figure 1. Crosslinking and Sonication Optimization.

Figure 1

(A) Minimally-sonicated, gastrula stage (stage 10) DNA from a 1% formaldehyde/1×PBS time course was resolved by 2% agarose gel electrophoresis. Onset of crosslinking is observed by the recovery of sonication-resistant, high molecular weight DNA (>1kb). (B) 60-minute crosslinked chromatin from gastrula stage embryos was fully sonicated for one to four rounds of 20 seconds each and resolved by 1.2% agarose gel electrophoresis. Three rounds of sonication balances optimal yield (90%) with maximal shearing (<1kb) of the crosslinked chromatin.