Table 1. Example of ChIP QPCR data analysis for one experiment.
| Goosecoid Promoter | average C(t) | standard deviation | ΔC(t)1 | ΔΔC(t) uninjected2 | fold change uninjected3 | % of input 4 |
|---|---|---|---|---|---|---|
| Input Uninjected | 29.7189 | 0.1963 | 4.4050 | 0.0000 | 1.0000 | 0.0472 |
| ChIP Uninjected | 34.1239 | 0.1043 | ||||
| Input myc-Fast1 | 30.9308 | 0.2736 | -3.1914 | -7.5964 | 193.5257 | 9.1351 |
| ChIP myc-Fast1 | 27.7393 | 0.1264 | ||||
| Input myc-Fast1 + Xnr1 | 31.0805 | 0.4056 | -3.1726 | -7.5766 | 191.0190 | 9.0168 |
| ChIP myc-Fast1 + Xnr1 | 27.9079 | 0.1602 | ||||
| Ef1α Control | average C(t) | standard deviation | ΔC(t)1 | ΔΔC(t) uninjected2 | fold change uninjected3 | % of input4 |
| Input Uninjected | 29.7538 | 0.2540 | 4.5108 | 0.0000 | 1.0000 | 0.0439 |
| ChIP Uninjected | 34.2646 | 0.4304 | ||||
| Input myc-Fast1 | 31.0208 | 0.1089 | 2.9121 | -1.5987 | 3.0287 | 0.1329 |
| ChIP myc-Fast1 | 33.9330 | 0.3681 | ||||
| Input myc-Fast1 + Xnr1 | 30.6189 | 0.2831 | 3.4157 | -1.0951 | 2.1363 | 0.0937 |
| ChIP myc-Fast1 + Xnr1 | 34.0346 | 0.1988 |
1
ΔC(t) = ChIP C(t) − Input C(t) (Taneyhill and Adams, 2008)
2
ΔΔC(t) = ΔC(t) − ΔC(t) uninjected (Taneyhill and Adams, 2008)
3
Fold Change = 2 [-ΔΔC(t)] (Taneyhill and Adams, 2008)
4
% of Input = 2 [-ΔC(t)] × (% I) (Frank et al., 2001)