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Deuterium uptake as a function of time for peptides V79 – H86, V195 – E205, V208 – G232 and I241 – D248 showing changes in deuterium uptake for PNP with: phosphate only (blue); the catalytically active Michaelis complex (red); DATMe-ImmH complex (green); and ImmH complex (orange). Highlighted in red in the peptide sequence are the residues implicated in nucleoside and/or binding.
