Skip to main content
. Author manuscript; available in PMC: 2011 Mar 9.
Published in final edited form as: Biochemistry. 2010 Mar 9;49(9):2058–2067. doi: 10.1021/bi902041j

Table 3.

Percent difference S(20,w) from PNP•PO4 (control) determined by analytical ultracentrifugation sedimentation velocity a

Complex Percent Difference S(20,w)
PNP + PO4 + Inosine 0.68 ± 0.05
PNP + PO4 + DATMe-ImmH 0.95 ± 0.38
PNP + PO4 + ImmH 2.21 ± 0.23
a

Each sample was centrifuged in triplicate with PNP•PO4 always included as an internal standard. The individual and average S(20,w) values were determined from runs summarized in Supporting Information Table 5. All complexes sediment more rapidly than PNP•PO4 alone (the internal control). The PNP•PO4•Inosine and PNP•PO4•DATMe-ImmH sediment within experimental errors of each other but both sediment significantly slower than PNP•PO4•ImmH.