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. Author manuscript; available in PMC: 2010 Mar 5.
Published in final edited form as: Arch Biochem Biophys. 2009 Feb 21;485(2):139–149. doi: 10.1016/j.abb.2009.02.008

Figure 4. Detection of binding of Prdx6 to oxidized liposomes by filtration analysis.

Figure 4

Prdx6 (80 µg/ml) in 40 mM PBS, pH 7.4, in the absence or presence of liposomes (100 µM total lipid) was processed through a YM-100 microfiltration system with a nominal cutoff of 100 kDa. The filtrate (F) and the retentate (R) were analyzed by SDS-PAGE with Coomassie blue staining (lanes 1–3 in each panel) followed by Western blot for Prdx6 (lanes 4–6 in each panel). Lanes 1 and 4 in each panel are the molecular mass markers; values for the Coomassie-stained gels are indicated at the far left and for the Western blots at the far right. The results are representative of three independent experiments. A. Prdx6 without liposomes. B. Prdx6 with non-oxidized liposomes. C. Prdx6 with oxidized liposomes.