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. Author manuscript; available in PMC: 2010 Mar 5.
Published in final edited form as: Arch Biochem Biophys. 2009 Feb 21;485(2):139–149. doi: 10.1016/j.abb.2009.02.008

Table 1.

Peroxidase activity and binding constants for wild type and mutant peroxiredoxin 6 proteins

Peroxidase Activity Binding to liposomes
H2O2
µmol/min/mg		 PLPCOOH
µmol/min/mg		 Liposomes (oxidized)
µmol/min/mg		 k1
107 M−1s−1 		Kd
µM
Wild type 6.20 ± 0.31 6.00 ± 0.17 5.95 ± 0.25 0.82 ± 0.2 0.23 ± 0.05
C47S 0 0 0 0.81 ± 0.1 0.22 ± 0.1
S32A 5.72 ± 0.20 0 0 ND ND
H26A 5.60 ± 0.20 0.28 ±0.09 ND ND ND
D140A 6.15 ± 0.25 5.25 ± 0.31 5.76 ± 0.38 1.2 ± 0.2 0.30 ± 0.09

Activity was measured at pH 7.4 in the presence of πGST. H2O2, 200 µM; PLPCOOH, 200µM; oxidized liposomes, 100 µM. PLPCOOH, 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine hydroperoxide. ND, not determined; zero, no signal detected. Results are mean ± SE for n=3.