Fig. 7.

Temperature optimum profile of BHPNP1. The temperature optimum of BHPNP1 was determined with reaction mixtures containing 1 mM guanosine in 50 mM sodium phosphate buffer, pH 8.0. PNPase (0.025 U) was added to initiate the reaction. After 10 min of incubation at temperatures between 30 and 90°C, the reaction was stopped by the addition of 0.5 ml of a 5 M NaOH solution. Guanosine conversion and guanine formation were analysed by HPLC