Table 2.
Total units | Specific activity | Percentage of recovery (%) | Fold purification | |
---|---|---|---|---|
Initial culture (intracellular) | 4,840 | 6.93 | 100.00 | 1.00 |
Lyophilised crude extract | 732 | 20.65 | 15.12 | 2.97 |
Ion exchange 1 (0–500 mM NaCl gradient) | 541 | 22.24 | 11.17 | 3.73 |
Ion exchange 2 (150–400 mM NaCl gradient) | 110 | 30.23 | 2.27 | 4.99 |