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Physical and kinetic characteristics of BHPNP1
| Parameter (units) | Inosine | Guanosine |
|---|---|---|
| Specific activity (U mg−1) | 30.23 | ND |
| K m (μM) | 236 | 206 |
| V max (mol s−1) | 4.76 × 10−6 | 2.03 ×10−9 |
| k cat (s−1) | 2.8 × 104 | 1.2 × 101 |
| Specificity constant (M−1 s−1) | 1.2 × 108 | 5.87 × 104 |
| pH Opt | 7 | |
| pH range (60%) | 5.7–8.4 | |
| Temp Opt (°C) | 70 | |
| Temp range (60%) (°C) | 32–74 | |
| Temp stability (t 1/2 at 60°C) (h) | 20.8 | |
| Temp stability (t 1/2 at 40°C) (h) | >200 | |
