Fig. 10.

Western blot analysis of nephrin and actin in isolated glomeruli from congenic rat substrains for α- and β-adducin. Renal glomeruli were isolated from 25–45-day-old male MHS, MNS rats and congenic substrains for α-adducin (NA), β-adducin (NB), and αβ-adducin (NAB). Five rats for each group were analyzed by Western blotting (10 µg protein/lane) using specific antibodies against nephrin and actin. The densitometric analysis is reported as arbitrary units. Data are mean ± SEM. *p < 0.05, **p < 0.01 vs. MNS. Figure S8 in supplementary data shows the Western blots for nephrin and actin of the individual renal glomerular preparations from rat substrains