Fig. 3.

Immunofluorescence analysis of podocyte proteins and α- and β-adducin in mouse kidney sections. Immunohistochemical expression of podocyte proteins from slit diaphragm (nephrin, Fyn) and foot process cytoskeleton (synaptopodin, α-actinin, ZO-1) and α- and β-adducin was evaluated in kidney preparations from 1.5-month-old male wild-type and β-adducin knockout mice. Five mice for each group were investigated. Magnification: nephrin ×1,000; synaptopodin ×630; α-actinin WT ×630; α-actinin KO ×400; ZO-1 ×400; Fyn ×1,000; α- and β-adducin ×400. Figure S2 in supplementary data shows the quantitative immunofluorescence analysis