Table 1.

SeqTrim accuracy evaluated with artificial sequences

	Vu+I			Vu+I+Vd
	Expected	Obs (enz)	Obs (-enz)	Expected	Obs (enz)	Obs (-enz)
Sequence size	362			412
Insert length	312	311.71	311.71	312	310.7	311.51
Insert-start point	51	51.18	51.2	51	51.2	51.2
Insert-end point	362	361.9	36.91	362	360.9	361.71
Rejected		3	0		1	0
Mistakenly processed		9	0		5	0
	Vu+I+pA			Vu+I+pA+Vd
	Expected	Obs (enz)	Obs (-enz)	Expected	Obs (enz)	Obs (-enz)
Sequence size	392			442
Insert length	312	311.32	311.33	312	310.72	311.33
Insert-start point	51	51.2	51.2	51	51.2	51.2
Insert-end point	362	361.52	361.53	362	360.92	361.53
Rejected		1	0		1	0
Mistakenly processed		3	0		5	0
	Vu+Au+I			Vu+Au+I+Ad+Vd
	Expected	Obs (enz)	Obs (-enz)	Expected	Obs (enz)	Obs (-enz)
Sequence size	375			438
Insert length	312	311.9	311.91	312	311.9	311.91
Insert-start point	64	64	64	64	64	64
Insert-end point	375	374.9	374.91	375	374.9	374.91
Rejected		2	0		0	0
Mistakenly processed		6	0		0	0
	Vu+Au+I+pA			Vu+Au+I+pA+Ad+Vd
	Expected	Obs (enz)	Obs (-enz)	Expected	Obs (enz)	Obs (-enz)
Sequence size	405			468
Insert length	312	311.53	311.53	312	311.53	311.53
Insert-start point	64	64	64	64	64	64
Insert-end point	375	374.53	374.53	375	374.53	374.53
Rejected		0	0		0	0
Mistakenly processed		0	0		0	0

Vu, 50 nucleotides preceding the BamHI restriction site of pBlueScript-FL. Vd, 50 nucleotides following the HindIII restriction site of pBlueScript-FL. I, a fragment of 312 nucleotides from Pinus pinaster genomic DNA. pA, poly-A tail of 30 A's. Au, upstream 5'-adaptor, containing the sequence GATCCGTTGCTGTCGTCG. Ad, downstream 3'-adaptor, containing the sequence CGGCCGCGTCGACAAGCT. 'Expected' corresponds to theoretical mean values for each set of artificial sequences. 'Obs (enz)' are the mean values obtained using SeqTrim with the cloning restriction sites specified. 'Obs (-enz)' are the mean values obtained using SeqTrim with no cloning restriction site specified.