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Dependence of the in vivo stimulation of HNF1 by wild-type DCoH and the mutant R31Q/K72E on the concentration of the expression vector pBJ5-DCoH. The basal HNF1 activity is set to 1. The experimental conditions are the same as described in Materials and Methods, except that the amount of vector was varied. The curves represent a typical experiment. Measurements were performed in duplicate.
