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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Mol Immunol. 2009 Dec 30;47(6):1278–1282. doi: 10.1016/j.molimm.2009.12.004

Fig.2.

Fig.2

Identification of the IRAK-1 motif is necessary for its interaction with VASP. (A) MAT-2 cells were transiently transfected with either pFlag-IRAK-1, pFlag-IRAK-1ΔC, or pFlag-IRAK-1(L167A/W168A) mutants. Equal amounts of lysate were harvested from the transfected cells and used to perform immunoprecipitation analyses using anti-VASP antibody. Co-immunoprecipitated protein complexes were resolved on SDS-PAGE and blotted with an anti-FLAG antibody (top right panel). A control anti-goat IgG was used to perform a similar immunoprecipitation study, and did not give a specific signal near the region of interest (data not shown). The expression levels of various Flag-IRAK-1 mutants within the cell lysates are indicated in the upper left panels and the levels of VASP in the cell lysates are shown in the bottom panels. (B) A diagrammatic illustration of various Flag tagged IRAK-1 full-length and deletion constructs used in the transfection studies.