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. Author manuscript; available in PMC: 2011 Mar 3.
Published in final edited form as: Cell Metab. 2010 Mar 3;11(3):231–238. doi: 10.1016/j.cmet.2010.01.005

Figure 4. Increased sympathetic activity in M3R-/- mice leads to low bone mass.

Figure 4

(A and B) ELISA assays of epinephrine content in urine of M3R-/- (A) and M3Rneuron-/- (B) as well as WT control mice. (C and D) qPCR analysis of Tph2 mRNA expression (C) and HPLC measurement of serotonin level in brainstem of WT and M3R-/- mice. (E and F) Histomorphometric analysis of vertebrae (E) and ELISA analysis of serum IGF-1 levels (F) of 24-week-old WT, Adrb2+/-, M3R-/-, and Adrb2+/-;M3R-/- mice. n = 6-10 per group; *P < 0.05 comparing to WT group; results are represented as mean ± SEM.