FIGURE 1.
Analysis of human milk OPN. A, Western blot analysis using a polyclonal OPN antibody. Lane 1, molecular mass standards. Lane 2, skim milk. Lane 3, OPN purified from milk. Lane 4, fraction I from B. Lane 5, fraction II from B. B, RP-HPLC of purified human milk OPN. Separation was performed on a Vydac C18 column connected to a GE Healthcare system by stepwise increasing concentrations of 75% propan-2-ol in 0.1% trifluoroacetic acid (TFA) (dashed line) at a flow rate of 0.85 ml/min. Proteins were monitored at 226 nm (solid line).