FIGURE 7.

ACER3 knockdown inhibits apoptosis in response to serum deprivation. A–C, HeLa cells were transfected with indicated siRNAs as described in Fig. 5 in regular medium containing 10% fetal bovine serum for 72 h before they were subjected to cell death detection ELISA (A), Western blot analysis for PARP cleavage (B), or caspase 3 cleavage (C). D–F, HeLa cells were transfected with indicated siRNAs for 24 h before they were switched to serum-free medium. At 48 h post-serum deprivation, the cells were subjected to cell death detection ELISA (D), Western blot analysis for PARP cleavage (E), densitometry of PARP (F), Western blot analysis for caspase 3 cleavage (G), and densitometry of caspase 3 (H). Numerical data represent mean values ± S.D. of three independent experiments. Image data represent one of three independent experiments with similar results. *, p < 0.05 versus the control (siCON or no siRNA).