FIGURE 2.

c-Src-KO mouse sperm undergo a capacitation-associated increase in tyrosine phosphorylation. A, capacitated and non-capacitated sperm extracts from Src-null (KO) and wild-type (WT) siblings were analyzed by Western blot with anti-pY (clone 4G10) antibodies. PVDF membranes were stripped and re-probed with anti-Src antibody (clone GD11), shown at the bottom. B, radioimmune precipitation assay and SDS extracts were further analyzed with a different anti-Src antibody (clone 32G6). A signal corresponding to c-Src was only detected in the WT siblings. Anti-tubulin and anti-actin immunodetections were used as loading controls.