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Okadaic acid does not induce phosphorylation events in sperm incubated under non-capacitating conditions or when the sperm are in the presence of the PKA inhibitor H-89. A, mouse sperm were incubated under conditions that support or do not support capacitation either in the presence or absence of 10 nm okadaic acid. B, sperm were incubated with 50 μm H-89 in the presence of 10 nm okadaic acid. All Western blots are representative of experiments repeated at least three times.
