FIGURE 6.
Okadaic acid rescues the inhibition of in vitro fertilization by SU6656. Sperm were capacitated in either the presence or absence of SU6656 (SU, 50 μm), okadaic acid (OA, 10 nm) or both. After capacitation, eggs were inseminated with 2.5 × 106 sperm/ml in 200-μl drops. As controls for the effect of the inhibitors on eggs, eggs were preincubated with inhibitors for 30 min and washed before insemination (where specified as SU eggs or OA eggs). Fertilization was assessed by microscopic visualization of pronuclei formation. Data represent mean ± S.E., n = 3; *, p < 0.01. At least 30 eggs were evaluated in each treatment.