TABLE 1.

Okadaic acid rescues sperm motility parameters affected by SU6656

Mouse sperm were incubated in the absence or presence of SU6656 (SU, 50 μm), okadaic acid (OA, 10 nm), or both inhibitors together (SU/OA) for 60 min in medium that either supports (with HCO₃⁻) (cap) or does not support capacitation (without HCO₃⁻) (non cap). After capacitation, sperm motility was examined using the CEROS computer-assisted semen analysis (CASA) system. Data represent mean ± S.E., n = 3. The effect of SFK and phosphatase inhibitors on mobility parameters of mouse sperm by CASA is shown. Mot, percent motility; VAP, velocity average path; VSL, velocity straight line; VCL, velocity curvilinear; prog, progressive motility.

Treatmenta	Mot	VAP	VSL	VCL	Prog
	%	μm/s	μm/s	μm/s	%
Non cap	75 ± 3.9	82 ± 4.9	63 ± 3.9b	129 ± 5.2b	27 ± 1.4b
Cap	81 ± 6.5	77 ± 4.4	51 ± 2.8	160 ± 3.3	22 ± 1.7
Cap+SU	59 ± 4.1b	23 ± 3.2b	17 ± 2.1b	43 ± 4.0b	2 ± 0.5b
Cap+SU/OA	67 ± 7.1	89 ± 9.1	71 ± 6.0	141 ± 5.3b	24 ± 2.1
Cap+OA	77 ± 6.9	60 ± 5.0	42 ± 2.5b	121 ± 4.6b	33 ± 1.5b

^a SU = 50 μm SU6656; OA = 10 nm okadaic acid.

^b p < 0.05 versus capacitated sperm.