FIGURE 1.

8-Cl-Ado accumulates as 5 major intracellular metabolites. A, HPLC/UV chromatogram of untreated JeKo cell extracts. B–C, HPLC/UV chromatogram and radiochromatogram of 10 μm 8-Cl-[³H]Ado-treated cells. JeKo cells were incubated with and without 10 μm 8-Cl-[³H]Ado (50 μCi) for 24 h. 8-Cl-[³H]Ado was purified by HPLC immediately before cell treatment (see “Experimental Procedures”). Cells were extracted with PCA and neutralized prior to HPLC analysis. For each chromatogram, extracts from an equal number of cells were injected on the column.