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MS/MS spectrum and proposed fragmentation pattern of succinyl-8-chloro-adenosine in negative ion mode. Succinyl-8-Cl-Ado was isolated and purified from 8-Cl-Ado-treated JeKo cells by PCA extraction and two HPLC separations (see “Experimental Procedures”). The eluate was dissolved in MeOH/water (1:1) for direct infusion to the qTOF.
