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. 2010 Jan 11;285(11):8022–8030. doi: 10.1074/jbc.M109.085803

FIGURE 3.

FIGURE 3.

Intracellular metabolism of 8-Cl-Ado as a function of dose and time. A, dose-dependent accumulation of 8-Cl-AMP, S-8-Cl-Ado, and 8-Cl-ATP. B, 8-Cl-AMP association with accumulation of its terminal metabolites (8-Cl-ATP and S-8-Cl-Ado) and depletion of ATP. For A and B, JeKo cells were incubated with 0, 1.25, 2.5, 5, 10, and 20 μm 8-Cl-Ado for 24 h. C and D, time-dependent accumulation of 8-Cl-Ado metabolites: 8-Cl-AMP, S-8-Cl-AMP, S-8-Cl-Ado, and 8-Cl-ATP. JeKo cells were incubated with 10 μm 8-Cl-Ado for up to 24 h. E, elimination curves for 8-Cl-ATP, S-8-Cl-Ado, and 8-Cl-AMP. After 24 h of preincubation with 10 μm 8-Cl-Ado, the medium was replaced with drug-free medium, and the metabolite concentrations were quantified at various time points up to 12 h. For all experiments, cells were extracted with PCA or 60% methanol prior to HPLC analysis. Data points are averages ± S.D. of three independent experiments.