FIGURE 4.

Succinyl-8-Cl-adenylate metabolism of multiple cell lines was associated with fumarate loss and initial fumarate concentration. A, accumulation of 8-Cl-ATP and S-8-Cl-Ado normalized to 8-Cl-AMP levels. B, accumulation of S-8-Cl-Ado normalized to S-8-Cl-AMP levels. C, accumulation of S-8-Cl-AMP normalized to 8-Cl-AMP. D, fumarate concentrations after 8-Cl-Ado treatment compared with the untreated controls. E, association between initial fumarate concentrations and fumarate change after 8-Cl-Ado treatment. Four MCL cell lines were continuously incubated with 10 μm 8-Cl-Ado for 6 h (D and E) or 24 h (A–E). Cells were extracted with 60% methanol and analyzed by HPLC. Data points are averages ± S.D. of three independent experiments. *, p < 0.05; **, p < 0.01.