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. 2009 Dec 30;285(11):8114–8121. doi: 10.1074/jbc.M109.063875

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Isomeric composition of retinal before and after light irradiation. Retinal composition before and after light stimulation shows that the main reaction in K296G/nPrSB is cis-trans isomerization of the 11-cis double bond similar to the wild-type rhodopsin. The mutant shows some all-trans isomers before light irradiation, presumably due to the excess chromophore added to reconstitute the pigment. It also shows some formation of 13-cis isomer after light irradiation; however, its contribution is small.