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Change of intrinsic fluorescence after flash light irradiation at 288 K of the wild type (a) and K296G/nPrSB (b). The data were fitted with a single exponential function, and the lifetime of retinal release was estimated. It is of note that the noise is much more prominent in K296G/nPrSB because of the difference in time resolution (1 s for the wild-type rhodopsin and 2 ms for K296G/nPrSB).
