FIGURE 2.
Effect on cytotoxicity of adding a single charged residue into the Phe clamp. Cells were incubated with PA heptamers at the indicated concentrations in the presence of 100 nm LFN-DTA. After 16 h, we measured the incorporation of [3H]leucine into protein over a 1-h period. A, [WT]6[F427K]1 (●), [WT]6[F427R]1 (▴), [WT]6[F427H]1 (▾), and [WT]7 (■). B, [WT]6[F427D]1 (●), [WT]6[F427E]1 (▴), and [WT]7 (■).