TABLE 1.
Ligand | Microtubule |
Kd |
|
---|---|---|---|
Microtubule co-sedimentation | Fluorescence anisotropy | ||
μm | |||
Tail944 | αβ | 0.45 ± 0.15 | |
Tail944 R907C-F | αβ | 0.65 ± 0.21 | 0.46 ± 0.02 |
Tail963 R907C-F | αβ | 0.56 ± 0.12 | 0.51 ± 0.07 |
Tail944 A905C-F | αβ | 0.65 ± 0.14 | 0.35 ± 0.03 |
Tail944 A905C-F Mutant A | αβ | 12.5 ± 2.2 | NDa |
Tail944 A905C-F Mutant B | αβ | 3.8 ± 1.4 | ND |
Tail944 A905C-F Mutant A+B | αβ | 16.7 ± 7.1 | ND |
Tail944 R907C-F | αsβs | 4.06 ± 2.9 | 3.17 ± 0.36 |
a ND, not determined.