TABLE 1.
Sequences of the AGT mutants used
| Mutant | Sequence |
|---|---|
| Sequences from residues 137–172 in hAGT (active site pocket)a | |
| hAGT | -NPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHE- |
| Ogt | -NPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHE- |
| hAGT-01 | -NPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLAHE- |
| hAGT-02 | -NPVPILIPCHRVIGRNGTMTGYAGGVQRKEWLLAHE- |
| hAGT-03 | -NPVPILIPCHRVIGRNGTMTGYSGGLAVKEWLLAHE- |
| hAGT-04 | -NPVPILIPCHRVIGRNGAVGNYSGGLAVKEWLLAHE- |
| hAGT-05 | -NPVPILIPCHRVVCSSGTMTGYSGGLAVKEWLLAHE- |
| hAGT-06 | -NPVPILIPCHRVVCRNGTMGNYSGGLAVKEWLLAHE- |
| hAGT-07 | -NPVPILIPCHRVIGRNGAVGGYSGGLAVKEWLLAHE- |
| hAGT-08 | -NPVPILIPCHRVVCSNGTVTNYSGGLAVKEWLLAHE- |
| hAGT-(C145S) | -NPVPILIPSHRVVCSSGAVGNYSGGLAVKEWLLAHE- |
| hAGT-03-(C145S) | -NPVPILIPSHRVIGRNGTMTGYSGGLAVKEWLLAHE- |
| Sequences from residues 94–136 in hAGT (DNA binding domain) | |
| hAGT | -FTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRG- |
| Ogt | -FQREV-WKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGS- |
| hAGT-(S113H) | -FTRQVLWKLLKVVKFGEVIHYQQLAALAGNPKAARAVGGAMRG- |
| Other mutants used | |
| hAGT-03-(S113H) | |
| hAGT-06-(S113H) | |
a Residues in hAGT that are changed to those in Ogt are shown underlined. The Cys145 acceptor site and its Ser145 mutant are shown in boldface type.