. 2009 Dec 21;285(11):8185–8195. doi: 10.1074/jbc.M109.045518

TABLE 2.

Summary of protection of E. coli cells from mutations and killing by MNNG, ENNG, and PNNG

Agent	NER status	AGT expressed	ED₂₀^a	Mutations (percentage of “no AGT” value^b)
Agent	NER status	AGT expressed	ED₂₀^a	G:C to A:T	T:A to C:G
			μg/ml	%	%
MNNG	NER⁻	None	0.4	100	100
MNNG	NER⁻	hAGT	4.5	3	19
MNNG	NER⁻	Ogt	3.4	6	<2
MNNG	NER⁻	hAGT-03	3.7	5	<2
ENNG	NER⁻	None	0.6	100	100
ENNG	NER⁻	hAGT	4.6	2	123
ENNG	NER⁻	Ogt	2.7	4	3
ENNG	NER⁻	hAGT-03	2.9	3	3
PNNG	NER⁻	None	0.6	100	100
PNNG	NER⁻	hAGT	>5	7	62
PNNG	NER⁻	Ogt	5	9	<2
PNNG	NER⁻	hAGT-03	>5	3	<2
MNNG	NER⁺	None	2.4	100^c	100^c
MNNG	NER⁺	hAGT	>10	2	125
MNNG	NER⁺	Ogt	10	4	<2
MNNG	NER⁺	hAGT-03	8.4	4	<2
ENNG	NER⁺	None	2.2	100^d	100^d
ENNG	NER⁺	hAGT	9	16	89
ENNG	NER⁺	Ogt	10	21	2
ENNG	NER⁺	hAGT-03	19	18	2
PNNG	NER⁺	None	3.3	100^e	100^e
PNNG	NER⁺	hAGT	10	53	83
PNNG	NER⁺	Ogt	10	60	<2
PNNG	NER⁺	hAGT-03	10	25	<2

^a There was no significant difference in survival between strains having different reporter genes but the same NER status (e.g. FC326 and FC218). Therefore, results for the survival are given as the mean of the two values. Results that are outside the range of doses tested are given as >5 or >10.

^b The mean of the results obtained with each dose of the alkylating agent shown in Fig. 4 and in supplemental Figs. 2 and 3 was expressed as a percentage of the mutations obtained in the cells lacking AGT.

^c In the absence of any AGT expression, the presence of NER reduced the MNNG-derived G:C to A:T mutations by 24% and the total A:T to C:G mutations by 22%.

^d In the absence of any AGT expression, the presence of NER reduced the ENNG-derived G:C to A:T mutations by 79% and increased the A:T to C:G mutations insignificantly ∼1.2-fold.

^e In the absence of any AGT expression, the presence of NER reduced the PNNG-derived G:C to A:T mutations by 92% and the total A:T to C:G mutations by 95%.