FIGURE 5.

GSH recovery in liver is enhanced in GSK-3β-silenced mice following APAP treatment. A, Cyp2e1 protein levels; B, GSH levels in total liver homogenate; C, GSH levels in isolated liver mitochondria. Control ASO (♦) and GSK-3β ASO treated (■) mice were given APAP (300 mg/kg, intraperitoneally). At the indicated times, mice were sacrificed, the liver removed and homogenized, and the cytoplasm and mitochondrial fractions were separated using differential centrifugation. Liver homogenate and isolated mitochondria were immediately placed in 5% metaphosphoric acid to prevent GSH oxidation. GSH levels were analyzed using HPLC with electrochemical detection as described under ”Experimental Procedures.“ Results are mean ± S.D. for three to five experiments. *, p < 0.05 versus control ASO-treated mice.