FIGURE 3.

Effects of SRT1720, SRT2183, SRT1460, and resveratrol on SIRT1 deacetylating activity with native p53 peptide substrate (Native Peptide 3 in supplemental Table S1) determined by HPLC. A, time course of SIRT1 reaction showing an increase in the deacetylated peptide product and a decrease in the acetylated peptide substrate. SIRT1 reactions were carried out with 5 nm SIRT1 under the conditions described under “Experimental Procedures.” B, time course of SIRT1 reactions shown as the percentage of conversion of native peptide substrate versus time. C, effects of the indicated compounds on SIRT1 activity. Error bars in B and C indicate S.D.