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. 2010 Jan 20;285(11):8375–8382. doi: 10.1074/jbc.M109.090266

FIGURE 2.

FIGURE 2.

An indispensable role of Mn-SOD for the cell-protective function of nuclear SIRT1. C2C12 cells expressing EGFP, SIRT1-EGFP (nuclear SIRT1), mtNLS-EGFP (cytoplasmic SIRT1), or H355Y-EGFP (dominant-negative SIRT1) were exposed to 100 μmol/liter Ant A for 8 h. A, the percentage of TUNEL-positive cells in EGFP-positive cells and LDH activity in the culture medium from four separate series of experiments are shown. B, representative immunoblots of C2C12 cells overexpressing each plasmid and quantitative analyses of the Mn-SOD expression. C, C2C12 cells expressing EGFP or SIRT1-EGFP were transfected with control siRNA or Mn-SOD siRNA and then exposed to 20 μmol/liter Ant A for 8 h. The percentage of EGFP-positive cells that were TUNEL-positive and the LDH activity in the culture medium from four separate series of experiments are shown. *, p < 0.05 versus EGFP-transfected cells. NS, no significant difference; LDH, lactate dehydrogenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.