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. 2010 Jan 4;285(11):8395–8407. doi: 10.1074/jbc.M109.038638

FIGURE 5.

FIGURE 5.

The MTK1/MKK3/6/p38α cascade mediates the GADD45β-driven C/EBPβ activation of the Col10a1 promoter. ATDC5 cells were co-transfected with 300 ng of the Col10a1-(−60/+52) promoter and 25 ng of expression vector encoding mouse C/EBPβ (Cβ) or GADD45β (45β), alone or together, and 50 ng of expression plasmid encoding either wild type MTK1 (Mw) or mutant MTK1K/R (Mm) (A); with 25 ng of expression vector encoding mouse C/EBPβ and 50 ng of MKK6KD (M6K), MKK7ala (M7a), MKK6glu (M6g), or MKK7glu (M7g) (lanes 4–7) or 25 ng of each expression vector encoding mouse C/EBPβ and GADD45β with increasing amounts (0, 25, 50, and 100 ng, indicated as factors of 0, 1, 2, and 4) of expression vector encoding MKK6KD or MKK7ala (lanes 8–16) (B); with 25 ng of each expression vector encoding mouse C/EBPβ and GADD45β with increasing amounts (0, 5, 25, and 50 ng, indicated as 0, 0.2, 1, and 2) of p38αapf (p38m) or Jnka1apf (Jnkm) expression vectors (lanes 4–11) (C); and 25 ng of each expression vector encoding mouse C/EBPβ and GADD45β or MKK3 (M3) and increasing amounts (0, 12.5, or 25 ng, indicated as 0, 0.5, and 1) of plasmid encoding MKP-1 (MKP) (D). Luciferase activities were normalized to pRL-TK reporter activities and shown as fold induction compared with control (empty vector). Means ± S.D. of triplicates from three independent experiments are shown. * indicates p < 0.01.