FIGURE 6.

Differential effects of threonine 235 on Col10a1 and C/EBP reporter activities. 25 ng of human C/EBPβ mutants (hCβ), threonine 235 to alanine (T235A), tyrosine 274 to phenylalanine (Y274F), and leucine 320 to arginine (L320R) were co-transfected in the absence or presence of 25 ng of expression vector encoding GADD45β (45β) using 300 ng of Col10a1-(−60/+52) (A) or pCEBP reporter vector (B) in a total of 375 ng/well in ATDC5 cells. Luciferase activities were normalized to pRL-TK reporter activities and shown as -fold induction compared with control (empty vector). Means ± S.D. of triplicates from three independent experiments are shown. *, p < 0.01. NS, not significant.