TABLE 2.
Effects of GADD45β on transactivation of Col10a1 (4.6 kb) or MMP13 (1.6 kb) promoter activity with ATF2, Fra2, and C/EBP family members
ATDC5 cells were transfected with reporter plasmid (300 ng) of Col10a1 (4.6 kb)-Luc (top three parts) or MMP13 (1.6 kb)-Luc (first, second, and fourth parts) and plasmids encoding Fra2 (first part), ATF2 (second part), or C/EBPα, C/EBPβ, or C/EBPδ (third and fourth part), alone or together with GADD45β in a total of 400 ng/well. Values of firefly luciferase activities are normalized to values of Renilla luciferase activities of pRL-TK and shown as -fold induction compared with control. Means ± S.D. from three or more independent experiments are shown. In the top part, p < 0.01 was considered as significant.
|
Col10a1 (4.6 kb) |
MMP13 (1.6 kb) |
|||
|---|---|---|---|---|
| Control | Fra2 | Control | Fra2 | |
| Control | 1.00 | 0.93 ± 0.08 | 1.00 | 2.20 ± 0.21 |
| GADD45β | 4.76 ± 0.83 | 4.35 ± 0.86 | 5.29 ± 0.62 | 13.33 ± 2.12a |
|
Col10a1 (4.6 kb) |
MMP13 (1.6 kb) |
|||
|---|---|---|---|---|
| Control | ATF2 | Control | ATF2 | |
| Control | 1.00 | 0.97 ± 0.28 | 1.00 | 0.93 ± 0.08 |
| GADD45β | 4.04 ± 1.30 | 9.30 ± 3.44b | 4.23 ± 0.64 | 7.03 ± 1.18a |
|
Col10a1 (4.6 kb) |
||||
|---|---|---|---|---|
| Control | C/EBPα | C/EBPβ | C/EBPδ | |
| Control | 1.00 | 18.77 ± 6.82 | 24.29 ± 10.12 | 17.32 ± 6.66 |
| GADD45β | 2.59 ± 0.41 (2.59) | 57.01 ± 21.91b (3.03) | 81.70 ± 31.18b (3.36) | 72.84 ± 29.25b (4.21) |
|
MMP13 (1.6 kb) |
||||
|---|---|---|---|---|
| Control | C/EBPα | C/EBPβ | C/EBPδ | |
| Control | 1.00 | 9.97 ± 2.06 | 7.26 ± 1.55 | 9.28 ± 2.45 |
| GADD45β | 2.64 ± 0.50 (1.92) | 20.80 ± 6.56a (2.06) | 14.84 ± 6.56a (2.04) | 21.75 ± 6.67a (2.34) |
a p < 0.01 for MMP13.
b p < 0.01 for Col10a1.