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. 2010 Jan 11;285(11):8408–8421. doi: 10.1074/jbc.M109.064386

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Oct25ΔPOU(273–301) antagonizes BMP4 activity in embryos. A, uninjected control (ctrl) embryos at tailbud stage. B, embryos show no dorsal structures upon dorsal injection of 100 pg of BMP4 RNA. C, embryos, co-injected with 100 pg of BMP4 and 1 ng of Oct25ΔPOU(273–301) RNAs together, display clear dorsal structures. D, gene expression analysis demonstrates that dorsal injection of BMP4 RNA leads to repression of dorsal genes, like Chd, Gsc, Dkk1, Xsox2, and Xsox3, and up-regulation of the ventral gene Xwnt8. This tendency is completely reversed when Oct25ΔPOU(273–301) RNA is co-injected. E, expression of the BMP-target Xvent-2 in embryos without injection (ctrl), after animal injection of hAlk-6 mRNA (1000 pg) alone, and hAlk-6 mRNA (1000 pg) in combination with Oct25ΔPOU(273–301) (500 pg). The ectopic activation on the dorsal side is not affected by Oct25ΔPOU(273–301). F, expression of Xvent-2 in embryos without injection (ctrl), after animal injection of BMP4 mRNA (1000 pg) alone, and BMP4 mRNA (1000 pg) in combination with Oct25ΔPOU(273–301) (500 pg). The ectopic activation of Xvent-2 is inhibited by Oct25ΔPOU(273–301).