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. 2010 Jan 11;285(11):8408–8421. doi: 10.1074/jbc.M109.064386

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Analysis of the effect after deletion of the region in Oct60, Oct91, and Oct4 corresponding to aa 273–301 in Oct25. A, uninjected control embryos at tailbud stage. B–D, ventral injections of 1 ng of Oct60ΔPOU(248–276) RNA (B), 1 ng of Oct91ΔPOU(264–292) RNA (C), and 1.5 ng of Oct4ΔPOU(177–205) RNA (D) lead to a dorsalized phenotype. The insets show embryos with partial double axis. E, qRT-PCRs reveal an up-regulation of dorsal genes in embryos injected with RNAs coding for Oct4ΔPOU(177–205), Oct91ΔPOU(264–292), or Oct60ΔPOU(248–276). F, qRT-PCRs of animal caps after injection with Oct91ΔPOU(264–292) or Oct60ΔPOU(248–276) RNAs also show stimulation of genes responsible for mesendoderm induction and body axis dorsalization.