TABLE 1.
Numbers of embryos injected with RNAs for different mutants and the respective numbers of phenotype occurrence
| Injected RNA (dose) | Total no. of injected embryosa | Occurrences of phenotype (%)a | Phenotypeb |
|---|---|---|---|
| Oct25ΔN (400 pg) | 65 | 58 (89%) | I |
| Oct25ΔC (400 pg) | 79 | 68 (87%) | I |
| Oct25PH (400 pg) | 52 | 48 (92%) | I |
| Oct25ΔPH (1000 pg) | 92 | 83 (90%) | II |
| Oct25ΔNLS (1000 pg) | 75 | 70 (93%) | II |
| Oct25ΔHox-(330–381) (1000 pg) | 103 | 89 (86%) | I |
| Oct25ΔPOU-(237–301) (600 pg) | 87 | 80 (92%) | II |
| Oct25ΔPOU-(237–301) (1200 pg) | 95 | 82 (86%) | II |
| Oct25ΔPOU-(273–301) (1000 pg) | 117 | 99 (85%) | III |
| Oct25ΔPOU-(250–301) (1000 pg) | 69 | 54 (78%) | III |
| Oct60ΔPOU-(248–276) (1000 pg) | 71 | 56 (78%) | III |
| Oct91ΔPOU-(264–292) (1000 pg) | 83 | 73 (88%) | III |
| Oct4ΔPOU-(177–205) (1500 pg) | 96 | 61 (64%) | III |
a Combined numbers of two or three experiments are shown.
b Phenotype I means failure of blastopore formation in injected embryos during gastrulation and loss of body axis formation during tailbud stage, which is similar to the phenotype after wild type Oct25 overexpression. Phenotype II means that the injected embryos did not show any essential difference from uninjected control embryos. Phenotype III means that the injected embryos were dorsalized.