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N-terminal sequences of hPRL mutants analyzed in this study. Sequences of the N-terminal/helix α1 for 3 natural ligands of the PRL/GH/PL family members are shown. The native N terminus of hPRL was iteratively modified to mimic that of oPL (elongation/mutation). Numbering corresponds to hPRL sequence, numbering for elongated mutants starts from −2 as indicated. The initial methionine Met(−3), found in recombinant proteins, is not indicated.
